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Literature summary extracted from
- A fluorescent assay suitable for inhibitor screening and vanin tissue quantification (2010), Anal. Biochem., 399, 284-292.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.92 | drug development | the highly sensitive fluorescent assay for the hydrolysis of pantothenate-7-amido-4-methylcoumarin as substrate to pantothenic acid and 7-amino-4-methylcoumarin could be a powerful tool for target validation and drug lead identification and characterization | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.92 | expressed in CHO cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.92 | 2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone | i.e. NSC 95397, from library of pharmacologically active compounds, LOPAC | Homo sapiens |  |
| 3.5.1.92 | 2-mercaptoethanol | reduced vanin-1 activity | Homo sapiens | |
| 3.5.1.92 | 5-(N,N-Hexamethylene)amiloride | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| 3.5.1.92 | 8-cyclopentyl-1,3-dipropylxanthine | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| 3.5.1.92 | beta-lapachone | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| 3.5.1.92 | dithiothreitol | vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol | Homo sapiens | |
| 3.5.1.92 | N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride | i.e. CGP-74514A hydrochloride, from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| 3.5.1.92 | roscovitine | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.92 | 13 | - |
pantothenate-7-amido-4-methylcoumarin | apparent Km value, soluble recombinant vanin-1, pH 7.7, 25°C | Homo sapiens | |
| 3.5.1.92 | 28 | - |
pantothenate-7-amido-4-methylcoumarin | apparent Km value, HPLC assay, pH 7.7, 25°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.92 | extracellular | the purified recombinant vanin-1 consists of a extracellular portion without a glycosylphosphatidylinositol linker | Homo sapiens | - |
- |
| 3.5.1.92 | soluble | an highly sensitive fluorescent assay using a novel fluorescent pantotheine derivative is used for characterization of a soluble version of human vanin-1 recombinant protein, identification of hits from high-throughput screening, and quantification of vanin pantotheinase acitivity in cell lines and tissues | Homo sapiens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.92 | dithiothreitol | important for vanin-1 activity, vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol. Optimal concentration: 0.4 mM | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.92 | 54000 | - |
size exclusion chromatography-multiangular light scattering (SEC-MALS) | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.92 | Homo sapiens | - |
- |
- |
| 3.5.1.92 | Mus musculus | - |
C57BI/6 mice | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.92 | recombinant enzyme,His- and Flag-tagged vanin-1 is purified over a 10 ml HisTrap Fast Flow column (a nickel affinity chromatography), 95% purity | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.92 | kidney | higher level in kidney than in liver, determined by RT-PCR | Mus musculus | - |
| 3.5.1.92 | liver | higher level in kidney than in liver, similar expression in lung, determined by RT-PCR | Mus musculus | - |
| 3.5.1.92 | lung | similar expression like in liver, determined by RT-PCR | Mus musculus | - |
| 3.5.1.92 | NCI-H292 cell | lung epithelial cells, transfected and selected for stable expression of human vanin-1 | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.92 | pantetheine + H2O | development of a highly sensitive fluorescent assay using a novel fluorescently labeled pantotheine derivative for high throughput screening for drug development and for quantification in various cell types and tissues | Homo sapiens | pantothenic acid + cysteamine | - |
? | |
| 3.5.1.92 | pantothenate-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | pantothenic acid + 7-amino-4-methylcoumarin | based on LC-MS analysis | ? | |
| 3.5.1.92 | pantothenate-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | pantothenic acid + 7-amino-4-methylcoumarin | based on LC-MS analysis, demonstrating that pantothenate-7-amido-4-methylcoumarin is hydrolyzed by vanin-1 at the amide-bond | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.92 | pantetheinase | - |
Mus musculus |
| 3.5.1.92 | pantetheinase | - |
Homo sapiens |
| 3.5.1.92 | vanin-1 | - |
Mus musculus |
| 3.5.1.92 | vanin-1 | belongs to the nitrilase superfamily, which contains the invariant catalytic triad residues: glutamate, lysine, and cysteine | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.92 | 25 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.92 | additional information | - |
greatly reduced activity observed at both acidic (pH 3.0) and basic (pH 10.0) conditions | Homo sapiens |
| 3.5.1.92 | 6.5 | 8 | - |
Homo sapiens |
| 3.5.1.92 | 7.5 | - |
assay at | Homo sapiens |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.92 | 0.00404 | - |
pH 7.5, 25°C | Homo sapiens | 5-(N,N-Hexamethylene)amiloride | |
| 3.5.1.92 | 0.0057 | - |
pH 7.5, 25°C | Homo sapiens | 2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone | |
| 3.5.1.92 | 0.0079 | - |
pH 7.5, 25°C | Homo sapiens | N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride | |
| 3.5.1.92 | 0.0095 | - |
pH 7.5, 25°C | Homo sapiens | beta-lapachone | |
| 3.5.1.92 | 0.0135 | - |
pH 7.5, 25°C | Homo sapiens | 8-cyclopentyl-1,3-dipropylxanthine | |
| 3.5.1.92 | 0.0176 | - |
pH 7.5, 25°C | Homo sapiens | roscovitine | |
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